General Cell Based Assays
Eurofins Discoverx PathHunter Human TREM1-DAP12 Signaling Reporter Cell-Based Assay Kit (Jurkat)
The PathHunter Human TREM1-DAP12 Signaling NFAT Reporter Stable Cell Line Assay (Jurkat) monitors the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation technology. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Apexbio Technology LLC Lactate-DH-Act-Assay-Kit-WST-8
Lactate Dehydrogenase Activity Assay Kit (WST-8) is widely used in biomedical research for assessing cell viability and cytotoxicity Originating from studies of cellular metabolism this assay targets the lactate dehydrogenase enzyme which plays a crucial role in the conversion of lactate to pyruvate through the glycolytic pathway The WST-8 compound produces a water-soluble formazan dye upon reduction by NADH reflecting enzyme activity It is commonly employed in in vitro assays with nanomolar to low micromolar sensitivity allowing precise measurement of cell damage or death Typical applications include use in various cell models animal studies and drug screening assays The assay is valuable for evaluating drug-induced cytotoxicity testing cell proliferation and examining metabolic activity Experimental concentrations typically depend on the specific research design ensuring flexibility and adaptability to diverse experimental setups
Apexbio Technology LLC Lactate-DH-Act-Assay-Kit-WST-8
Lactate Dehydrogenase Activity Assay Kit (WST-8) is widely used in biomedical research for assessing cell viability and cytotoxicity Originating from studies of cellular metabolism this assay targets the lactate dehydrogenase enzyme which plays a crucial role in the conversion of lactate to pyruvate through the glycolytic pathway The WST-8 compound produces a water-soluble formazan dye upon reduction by NADH reflecting enzyme activity It is commonly employed in in vitro assays with nanomolar to low micromolar sensitivity allowing precise measurement of cell damage or death Typical applications include use in various cell models animal studies and drug screening assays The assay is valuable for evaluating drug-induced cytotoxicity testing cell proliferation and examining metabolic activity Experimental concentrations typically depend on the specific research design ensuring flexibility and adaptability to diverse experimental setups
Eurofins Discoverx PathHunter eXpress CCR7 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0195E1CP0M)
The PathHunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR7 and EA-tagged β-Arrestin-2. Activation of CCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter AGTR1 Bioassay Kit, 2-Plate. (93-0312Y2-00125)
The PathHunter AGTR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTR1 and EA-tagged β-Arrestin-2. Activation of AGTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress ADRB3 DLD1 GPCR Assay, 10-Plate Kit. (95-0182E13CP25L)
The cAMP Hunter ADRB3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB3 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB3, a naturally Gs coupled GPCR. Upon activation of ADRB3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human GRO- ASY
The Meso Scale Discovery U-PLEX Human GRO-alpha Assay Kit is a multiplex immunoassay for measuring GRO-alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and precision Requiring 50 L or less sample volume it can be automated on MSD platforms The kit includes a 96-well plate with capture and detection antibodies calibrators and controls for standard curve generation and assay validation It allows simultaneous measurement of GRO-alpha and other biomarkers with high reproducibility